IN SILICO STUDY OF APTAMER SPECIFICITY FOR DETECTION OF ADENOSINE TRIPHOSPHATE (ATP) AS BIOSENSOR DEVELOPMENT FOR MITOCHONDRIA DIABETES DIAGNOSIS

Diabetes Mellitus (DM) is characterized by increased blood glucose levels. It generally caused the pancreas' inability to produce insulin due cell damage or resistance. Due inhibition of adenosine triphosphate (ATP) production, which essential for secretion, one clinical pathology this complication secretion dysfunction. Common methods sugar diagnostics cannot distinguish mitochondrial diabetes and can lead medication errors. Furthermore, an approach was developed through ATP biomarkers using electrochemical biosensor with help aptamer. However, it remains unknown precisely how where molecular interactions between modified aptamer occur. Simulations were conducted in study 100 ns silico amber18 computer program determine stability interaction specificity aptamer-ATP compared ADP AMP. The results showed that significant are three hydrogen bonds G7, G8, A24. discovered complex had moderately good better potential than According RMSD, RMSF, binding energy profiles, system still searching best conformation, necessitating a longer simulation time additional studies optimize system. As result, reach stable state more accurate calculation, hence, interpreted according real applications.